Higher Plants - Leaves and Petals
Cryo-SEM: Tanacetum vulgare (Tansy) flower stem
Cryo-fractured surface with spiral apical openings (phloem and xylem vessels) for fluid and gaseous transport. Note the in tact chloroplast and cross-section of the cell wall in the bottom right image. Fracture temperature: -145ºC. Coating: 2-3 nm Pt.
Images courtesy of FEI.
Cryo-SEM: Lavender leaf
Shows branching trichrome structures (blue) which help to protect the plant from predation. On the surface of the leaf are well preserved stomata (with their typical sausage-shaped guard cells) through which gaseous exchange occurs. Characteristic lavender oil droplets (red) can be seen on the leaf surface.
The specimen was prepared using a PP3000T cryo preparation system.
Cryo-SEM: water droplet dispersion on sycamore tree leaves
A cryo-SEM study looking at wetting characteristics of waxy plant leaf surfaces and to measure contact angle between the water droplet and leaf.
The specimen was prepared using a PP3010T
fitted to an FEI Inspect S.
Cryo-SEM: Soya bean leaf
Left: cold-fractured and coated with Pt. Right: underside of leaf showing stomatal guard cells. Below right: pectic strands which interconnect palisade cells in many plant species