Cryo-SEM: cocci type of bacteria
Coccus (plural cocci) is a bacterium that has a spherical, ovoid, or generally round shape. It is one of the three distinct bacterial shapes, the other two being bacillus (rod-shaped) and spiral-shaped cells.
Identification of Herpes virus in fixed cryo-fractured, cryo-protected cells.
Herpes infected cells were fixed and either prepared for the Transmission Electron Microscope (TEM) (A) or cryo-protected with 20% glycerol, frozen in liquid N2 slush, transferred to the preparation chamber and cold fractured prior to being sputter coated with Cr. The specimen was viewed in its frozen hydrated state at -150°C in a Field Emission Scanning Electron Microscope (FE-SEM) (B).
A. TEM Micrograph of an infected cell’s nucleus (N). Viral particles (arrows) are seen in association with the nuclear membrane.
B. Cryo FE-SEM of a similar field to that seen in (A). Viral particles (arrows) are clearly seen emerging through the nuclear pores of the nucleus (N).
Images by courtesy of Cameron Ackerley, The Hospital for Sick Children, Toronto.