Posts from March 2012

Competition winner announced

Following our recent competition to find the best short application note and/or image series completed using Quorum Technologies preparation instruments, we’re pleased to announce we have a winner!

The winner is Guohui Lin, a senior electron microscopist working at the Experimental Centre of South China Normal University, located in Guangzhou, Guangdong Province.

His specimens were part of a study looking at the effects of anti-cancer medicine, using a Quorum Technologies K750X Peltier-Cooled EM Freeze Dryer and a Q150T ES Turbo-Pumped Sputter and Carbon Coater.

Living mammary cells were cultured on polystyrene plates and the cells infected with bacteria. The cells were then chemically fixed in 2.5% gluteraldehyde in a fridge for six hours. 

After this, the specimens were rinsed in changes of phosphate buffer and rapidly frozen in slushy liquid nitrogen, generated in the K750X. Specimens were then freeze-dried in the K750X for six hours.

The dried specimens were mounted on stubs and sputter-coated with platinum (Pt) using the Q150T ES. Observation and imaging was then carried out using a Carl Zeiss ULTRA55 Field Emission Scanning Electron Microscope (FE-SEM)..

The competition marked forty years since the launch of Quorum Technologies’ first sputter coating and critical point drying systems in 1971. Other image submissions will be available in the Image Gallery soon. Guohui's images above show tumour cells infected with bacteria, freeze-dried and sputter-coated.