The PP3000T is a highly automated, easy to use, column-mounted, gas-cooled cryo preparation system suitable for most makes and models of SEM and FE-SEM. The PP3000T has all the facilities needed to rapidly freeze and transfer specimens. The cryo preparation chamber is turbomolecular pumped and includes tools for cold fracturing, controlled sublimation and specimen coating. The specimen can then be transferred onto a highly stable SEM cold stage for observation. Cold trapping in the cryo preparation chamber and SEM chamber ensures the whole process is frost free.