The GloQube® Plus is a cost-effective, compact and easy to use glow discharge system, designed to fulfil the needs of laboratories with TEM.
| Surface state || Charge || Atmosphere || Typical applications |
| Hydrophilic || Negative || Air || Hydrophilisation and cleaning of carbon coated TEM grids |
| Hydrophilic || Positive || Air* || Nucleic acid adhesion to carbon films |
| Hydrophobic** || Positive || Alkylamine || Controlled orientation and improved adhesion of negatively charged proteins, antibodies and nucleic acids |
| Hydrophobic** || Negative || Methanol |
Controlled orientation and improved adhesion of positively charged protein molecules (e.g. ferritin, cytochrome c)
* Air followed by post-treatment with magnesium acetate by the user.
** Hydrophobic, as noted above, may represent a less hydrophilic sample of less than 90 degrees contact angle.
Twin chambers prevent cross contamination
The GloQube® Plus uses a single door with two independent vacuum chambers and adjustable sample stages. The in-air chamber is for simple glow discharge hydrophilic applications, while the in-vapour chamber is designed for hydrophobic (negative or positive) conversions, typically using reagents such as methanol and amylamine. By utilising purge and flush cycles, we ensure contamination from the vapour chamber* does not affect the in-air chamber.
* For health and safety reasons when using the in-vapour chamber with chemicals the pump exhaust must be vented to a suitable external extraction system
The automatic valve system allows greater control over the introduction of chemical vapour into the chamber. This results in reproducibility and repeatability of processing. The self-contained nature of the septum-sealed
chemical vials and the delivery system ensures minimal user contact and a high level of operator safety.**
** Chemical preparation and disposal should be carried out in a suitable fume hood.
Easy sample loading
Each of the twin chambers can accommodate two 25 x 75 mm glass
microscope slides or TEM grid holders. Loading could not be easier using a drawer-style chamber door and specimen stages. The stages are height adjustable and fitted with removable glass slide holders. The door and stages can be completely removed for convenience and to allow easy access for chamber cleaning.
The GloQube® Plus is an automated system, which has a recipe driven process control user interface for ease of use. Filtered inlets ensure that delicate samples, such as carbon coated TEM grids, are not contaminated with particles or dust. The GloQube® Plus requires a single vacuum pump working in the 0.1 to 1 mbar range and has a typical pump downtime to operational vacuum of 60 seconds and a total cycle time in-air of usually less than two minutes*. A 750 mm flexible stainless vacuum hose is supplied with the GloQube® Plus.
* Using a 30 second glow discharge process
This product is for Research Use Only.