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PP3005 SEMCool Non-Airlock Cooling System for SEM and FIB/SEM

The SEMCool is based on the PP3006 CoolLok but without the PP3004 QuickLok components. It is designed for cryogenic applications where airlock exchange of specimens into the microscope is not required.
Key features
  • Temperature range down to -190°C with stability better than 0.5°C
  • Off-column cooling with all-day runtime between fills
  • Independent cooling of cold stage and cold trap
  • Upgrade path to PP3006 CoolLok
  • Extended warranty option
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Cold stage and cold trap

A highly stable and thermally isolated nitrogen gas-cooled cold stage attaches to the SEM stage. The location and shape of the cold trap is tailored to suit the internal geometry of the SEM. Both cold stage and cold trap are capable of reaching temperatures down to -190°C with a stability of <0.5°C. For easy specimen exchange an interlocked LED chamber light is fitted.

The cold stage connects to the SEM stage using an adaptor and has a dovetail fitting to accept a cryo specimen holder. When not in use the cold stage is uncoupled and stored within the chamber with the gas and electrical fittings connected.

Cooling dewar, trolley and controller

The cold stage and cold trap are cooled by a floor-mounted, vacuum isolated 21 litre dewar and heat exchanger assembly which at normal operating temperatures can run for up to 24 hours between fills. The gas lines between the dewar and the SEM interface are vacuum isolated for maximum thermal efficiency.

The cooling dewar is located on a floor-mounted trolley which also houses the temperature and gas control system.

Using the SEMCool

The SEM (or FIB/SEM) is vented and the specimen/specimen holder is manually located on the cold stage. The microscope is pumped down and the SEMCool cooled to the required working temperature. To exchange specimen warm the cold stage to above 0°C and vent the SEM.

The PP3005 is only recommended for applications where infrequent exchanges of specimens is expected. For greater specimen throughput an airlock into the SEM or FIB/SEM is essential. For these applications please see: PP3006 CoolLok and PP3010T Cryo Preparation System.


The SEMCool requires a rotary pump to periodically evacuate the vacuum isolated lines (see Ordering Information).  

This products is for Research Use Only. 

PP3005 SEMCool non-airlock cooling system

Consisting of: Nitrogen gas cooled stage with heater and sensor and cold trap with temperature sensor. Precise temperature control with a range down to 190°C, 21 litre liquid nitrogen dewar with trolley, heat exchanger and LED chamber light. Pump fittings (see: Pumping below). Temperature and nitrogen gas flow controller mounted on the dewar trolley. Specimen holders. 3 x AL200077B specimen shuttle (to hold 10 mm Ø cryo stubs), 12434 blank specimen shuttle, 20720 specimen shuttle with holding clips, E7402 blank 10 mm Ǿ stubs (packet of 10), 5 x E7449-7 multi-purpose specimen stubs. Note: other holders are available from Quorum. Specimen mounting compounds (colloidal graphite and Tissue-Tek ®). 


The PP3005 requires a rotary pump for periodically evacuating the vacuum isolated gas lines.

13034. Rotary pump. 5 m3/hr Pfeiffer Duo 6 rotary vacuum pump with oil mist filter.

Options and accessories

13296. Sircal in-line gas dryer. Helps to reduce water content of nitrogen gas supply if an external gas source is used.

The range of specimen holders available with the PP3010T can be used. Please contact Quorum for a complete list or see: PP3010T specimen holders

For a full cryo preparation/transfer system see: PP3010T