Single-cell organisms like algae are one of the most fragile specimens and require very careful and complex preparation before SEM imaging.
Choosing the right method and the support for such a specimen is key to successful imaging.

Here we present an outcome of using Aclar support for freshwater life imaging. The sample was subjected to fixation with 2.5% glutaraldehyde, rinsing with 1% sodium phosphate buffer and dehydration in a series of ethanol concentrations before critical point drying (Quorum K850) and coating with platinum-8nm in Q150V Plus.