Knowledge Centre


Application Notes




November 9, 2022 @ 11:00 am – 11:30 am GMT

Coffee Talks with Dr Anna Walkiewicz – Coating for SEM. How to choose vacuum level, metal and thickness

Coating for SEM. How to choose vacuum level, metal and thickness. In this Coffee Talk Dr Anna Walkiewicz will guide you through metal coating for SEM imaging. During the tutorial, […]
More Information
October 26, 2022 @ 11:00 am – 11:30 am BST

Coffee Talks with Dr Anna Walkiewicz – Mounting samples and coating; how to do it correctly

In this Coffee Talk you will learn about techniques used to attach samples to SEM stubs, different materials and accessories that could be used to expose parts of interest in […]
More Information
October 12, 2022 @ 11:00 am – 11:30 am BST

Coffee Talks with Dr Anna Walkiewicz – Critical Point Drying: A guided tour through a complex process

In this series of mini-talks, Dr Anna Walkiewicz will be describing the process of sample preparation by breaking it down into simple steps, aiming to solve common problems, while looking […]
More Information

Application Notes


PP3010 Cryo-SEM/Cryo-FIB/SEM Preparation System

The PP3010 is a highly automated, easy-to-use, column-mounted, gas-cooled cryo preparation system suitable for most makes and models of SEM, FE-SEM and FIB/SEM.

The PP3010 has all the facilities needed to rapidly freeze, process and transfer specimens. The cryo preparation chamber is turbomolecular pumped and includes tools for cold fracturing, controlled automatic sublimation and sputter coating. After processing, the specimen is transferred from the cryo preparation chamber onto a highly stable SEM cold stage for observation. Cold trapping in the cryo preparation chamber and SEM chamber ensures the whole process is frost-free.
Download Brochure
Our Full Range of Sputter Coaters

The Q Plus Series

The Q Plus Series is the latest version of Quorum’s internationally acclaimed, market-leading range of sputter and carbon coaters. They are increasingly used in high-resolution microscopy, where ultra-fine coating is a must.

Sputter coating is the standard method for preparing non-conducting or poorly conducting specimens prior to observation in a scanning electron microscope (SEM). Quorum offer low-cost, rotary-pumped sputter coaters for depositing non-oxidising metals – such as gold (Au) and platinum (Pt) – and turbomolecular-pumped coaters, suitable for both oxidising and non-oxidising metals, such as chromium (Cr).
Download Brochure

GloQube® Plus Glow Discharge System for TEM Grids and surface modification

The GloQube® Plus is a cost-effective, compact and easy to use glow discharge system, designed to fulfil the needs of laboratories with TEM. The primary application of the GloQube® Plus is to modify the surface of TEM grids in a way that it meets requirements for successful imaging of a variety of macromolecules. Integrated into one system, the two chambers enable the user maximum flexibility to choose which sample preparation technique they want to use: glow discharge in-air or in-chemical vapour, without downtime for cleaning or the risk of contamination and loss of samples.
Download Brochure

K850 Critical Point Dryer

The K850 combines versatility and ease of operation. Built-in thermo-electric heating and adiabatic cooling allow precise temperature control. The vertical pressure chamber (32 mm diameter x 47 mm) has a side viewing port, which gives a clear view of the liquid meniscus during filling. A magnetic stirrer ensures optimal mixing and exchange of process fluids.
Download Brochure

Production Image Library

Cryo-SEM Images

Higher Plants – Floral Structures

Cryo-SEM: Forget-me-not (Boraginaeace) plant petal surface with pollen

Prepared using a Quorum Technologies PP3000T Cryo-SEM Preparation System attached to a Carl Zeiss Sigma FE-SEM.

Images courtesy of the Image and Analysis Unit at the School of Pharmacy and Biomolecular Sciences, University of Brighton, and Carl Zeiss SNT.
More Information
Higher Plants – Floral Structures

Cryo-SEM: plant pollen

Examples of pollen prepared by cryo-SEM, including “mother and baby”
More Information
Higher Plant – Roots

Cryo-SEM: frozen hydrated root hairs

One of the most delicate structures borne on higher plants is the root hair. This is simply a projection of the outer tangential wall of the epidermis of the root, and has a mechanical function as well as a physiological one.

Because root hairs are over 90% water, conventional preparation methods, such as air or critical point drying, always result in considerable collapse, distortion and shrinkage of these structures. Low-temperature SEM (cryo-SEM), on the other hand, provides good preservation of structure without any collapse.

Root hairs illustrated here still have a thin layer of water (ice) on their surface, verifying their true frozen hydrated nature. If the ice proves to obscure important detail, it can be removed during the cryo-SEM process by careful sublimation (carefully raising the specimen temperature for a limited time).

Bar: 50 µm (inset: 25 µm)
More Information
Higher Plant – Roots

Cryo-SEM: starch granules in maize

Cryo-SEM prepared specimen: rapidly frozen in slushy nitrogen, fractured at -140ºC, sublmated at -90ºC for 3 minutes and sputter coated with platinium.

Image courtesy of Tongji University, Shanghai.
More Information

Freeze Dryer Images

Freeze-dried apple

Freeze-dried apple

Examples of freeze dried apple.

Images courtesy of Dr. Eric Curry, USDA, ARS, Tree Fruit Research Laboratory, Wenatchee, WA USA.
More Information
Freezer dryer images

Human monocyte derived macrophages

Freeze-dried using Quorum K775X and coated with carbon.

Images courtesy of Dr Jeremy Skepper of the Cambridge Advanced imaging Centre, University of Cambridge.
More Information

Product Videos

Length: 5:47

Quorum PP3010 Cryo Sample Preparation Process

Simplicity is key and Quorum’s Technical Director Robert Morrison, along with help from Dr Chris Parmenter at the nmRC, takes you through the simple steps of processing a sample using the PP3010 Cryo.

Length: 6:47

Quorum PP3010 Cryo Benefits

Why choosing the PP3010 Cryo System is the only choice for Cryo EM sample preparation.

Length: 11:18

Quorum Q150V Plus

Scanning electron microscope sample preparation with the Q150V Plus. Optimised for high-vacuum applications, with an ultimate vacuum of 1×10-6mbar, it can be used for either ultra-fine sputter coating or quality carbon coating.

Length: 2:26

GloQube Plus

The GloQube® Plus is a cost-effective, compact and easy to use glow discharge system, designed to fulfil the needs of laboratories with TEM.

Length: 1:59

Quorum Technologies Ltd

Specialists in EM sample preparation


Judges House, Lewes Road,
Laughton, East Sussex.

+44 1323 810981

[email protected]


Working Hours

8.30 – 5.00 pm Mon – Thu
8.30 – 4.00 pm Fri

Follow Our Socials

Keeping in contact with our customers via social platforms as well as traditional telephone and email.