Sample Preparation Archives - Quorum Technologies Ltd

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In this series Dr Anna Walkiewicz, the Quorum Application Specialist, will talk about different processes within the sample preparation workflow and breaking them down into simple steps, solving common problems and looking at how to improve efficiency and efficacy.

The first coffee talk, The Scary Vacuum, will be addressing issues regarding the vacuum: why do we need to consider if our samples are sensitive to the change of pressure and how they will behave in the vacuum environment.  Dr Anna Walkiewicz will be giving advice how to prepare samples in the correct way, so we see their natural appearance rather than an outcome of the pressure change modifying their morphology. Register here Coffee Talks with Dr Anna Walkiewicz – The Scary Vacuum 14th September – Quorum Technologies Ltd

 


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See a piece below from our customers at the Cambridge Advanced Imaging Centre at Cambridge University.

Cambridge Advanced Imaging Centre (CAIC) forms the hub of a network that draws together imaging activity across the whole University to serve the biomedical community. Many powerful modern imaging techniques have recently been developed, yet their exploitation in biology and biomedical research has largely been prevented by the high cost of their translation into widely available instruments and the specialist skills required for their use, including handling the vast volumes of data they generate. As a consequence, instruments developed in laboratories within specific Cambridge research programmes are often not available to the University’s wider biomedical research community.

Our Verios 460 SEM is equipped with a Quorum PP3010T cryo-SEM preparation system. Cryo-SEM allows imaging of soft, hydrated biological or materials samples in a frozen state. One of the major advantages of cryo-SEM is that samples can be imaged with minimal preparation without the need of any prior fixation or dehydration steps; a disadvantage is that sample throughput is fairly low compared to other methods. Specimens are mounted on an appropriate cryo-SEM shuttle, are plunge-frozen in slushed liquid nitrogen and then transferred to a cooled prep stage. Once on the prep stage, samples can be fractured, sublimed to remove any residual ice, and coated to improve contrast and conductivity (CAIC routinely uses a platinum target). Then, the sample is transferred onto the SEM cryo-stage for imaging. As cryo-SEM requires about 2h of run-up and run-down time of the system apart from the actual imaging time, we recommend half-day or whole-day bookings.

Some images below from their cryo-SEM

 


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Dr Anna Walkiewicz, the Quorum Technologies Application Specialist, is running a series of informative and educational webinars across this autumn looking at all aspects of sample preparation. The first talk is on 12th September at 11am BST, until 11.30am BST.

In this series, Dr Walkiewicz will be tackling different processes within the sample preparation process and breaking it down into simple steps, solving common problems and looking at how to improve efficiency and efficacy.

  • CT 01-The Scary Vacuum – 12th September – How will samples behave in the vacuum?
  • CT 02-Dehydration 26th September – Why is it so important? Why do we dehydrate samples? Do we need anything before dehydration? Means of tissue fixation
  • CT 03- Critical Point Drying 10th October – is it really so complex?
  • CT 04- Mounting samples 24th October – exposing the most relevant parts. Coating – why do we coat samples?
  • CT 05- Coating for SEM 7th Nov – What do we use for coating and when? Vacuum level, metal choice, thickness

To register to watch these events, please visit our events page and fill out the form


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